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Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system
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10.1118/1.2940603
/content/aapm/journal/medphys/35/7/10.1118/1.2940603
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/35/7/10.1118/1.2940603

Figures

Image of FIG. 1.
FIG. 1.

The hand-held probe consists of a probe head, a source fiber bundle, and a collection fiber bundle. The solid dots on the probe represent the six illumination points.

Image of FIG. 2.
FIG. 2.

(a) Schematic of the instrumentation for the hand-held probe based frequency domain ICCD optical imager. The hand-held probe is coupled to the laser source and ICCD detector for illumination and detection, respectively. (b) Experimental setup of the hand-held probe based optical imager for obtaining fluorescence measurements from a slab phantom. During actual experiments, the acrylic container was filled with 1% Liposyn solution as well.

Image of FIG. 3.
FIG. 3.

Target’s location with respect to the slab phantom for different experimental cases (A-E) shown as white hollow circles. The same locations were used for different target depths of 1.5 and deep, during the heterogeneous phantom studies. The gray shaded circles represent the six simultaneous points of illumination. The white rectangular box on the phantom surface represents the location of the hand-held probe during imaging.

Image of FIG. 4.
FIG. 4.

Iso-surface contour plots of the 3-D image reconstructions presented in the and planes for heterogeneous phantoms under 1:0 fluorescence contrast ratio at a fixed target location . The hollow circle in each plot represented the true target location and the irregular solid region represents the reconstructed target location. A cutoff value (selected based on the first break point of histogram plot of ) was applied to iso-surface contour plots. Only along the depth is represented in the plot, although the total depth, .

Image of FIG. 5.
FIG. 5.

Two-dimensional surface contour plots of detected fluorescence amplitude on the imaging plane of heterogeneous phantoms under 1:0 fluorescence contrast ratio, at different target depths of [rows (a)] and deep [rows (b)], and different experimental target locations (cases A–E). The hollow circle in each plot represents the true target location. The fluorescence intensity of surface signal was indicated by the color map scale in the bottom of plots. In order to compare the signal strength of different experimental cases, the measurements were not referenced, but all plots were generated using the same color map scale.

Image of FIG. 6.
FIG. 6.

Iso-surface contour plots of the 3-D image reconstructions presented in the and planes for heterogeneous phantoms under 1:0 contrast ratio, different target depths of (a) , and (b) . The five columns in each row represent the five different target locations (A–E). The hollow circle in each plot represented the true target location and the irregular solid region represents the reconstructed target location. A cutoff value (selected based on the first break point of histogram plot of ) was applied to iso-surface contour plots. Only along the depth is represented in the plot, although the total depth, .

Tables

Generic image for table
TABLE I.

Optical properties of fluorescent target and nonfluorescent background for all phantom studies.

Generic image for table
TABLE II.

The target location details for different experimental cases (A–E) at two different target depths.

Generic image for table
TABLE III.

Details of 3-D image reconstructions using simultaneous and sequential illumination for the perfect uptake (1:0) phantom study at target 3-D location of (, , and ).

Generic image for table
TABLE IV.

Details of 3-D image reconstructions for the perfect uptake (1:0) phantom study under a target depth of .

Generic image for table
TABLE V.

Details of 3-D image reconstructions for the perfect uptake (1:0) phantom study under a target depth of .

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/content/aapm/journal/medphys/35/7/10.1118/1.2940603
2008-06-26
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/35/7/10.1118/1.2940603
10.1118/1.2940603
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