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High-speed dynamic 3D photoacoustic imaging of sentinel lymph node in a murine model using an ultrasound array
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10.1118/1.3168598
/content/aapm/journal/medphys/36/8/10.1118/1.3168598
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/36/8/10.1118/1.3168598
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic of the high-speed 3D photoacoustic imaging system. Inset (a). Optical illumination pattern on the skin surface.

Image of FIG. 2.
FIG. 2.

Noninvasive in vivo photoacoustic mapping of the sentinel lymph node in a mouse. (a) Photograph taken before photoacoustic imaging. BT, black tape, was used to guide the initial positioning. (b) Control photoacoustic MAP image acquired at laser wavelength before Evans blue injection. BV, blood vessel. The color bar represents optical absorption. (c) Photoacoustic MAP image acquired at after Evans blue injection. SLN, sentinel lymph node. LV, lymph vessel. (d) Control photoacoustic MAP image acquired at . (e) Photoacoustic MAP image acquired at after Evans blue injection. (f) Composite photoacoustic MAP image. (g) Composite photoacoustic B-scan image corresponding to the dotted line in (c), showing the depth of the SLN. SK, skin surface. (h) Composite 3D photoacoustic image. (i) Photograph taken with skin removed after photoacoustic imaging.

Image of FIG. 3.
FIG. 3.

Evans blue dynamics in the SLN of a mouse monitored by photoacoustic imaging. (a) Control photoacoustic MAP image in a mouse (mouse 2) acquired before Evans blue injection. BV, blood vessel. The color bar represents optical absorption. [(b)–(e)] Photoacoustic MAP images acquired at , , , and , respectively, after Evans blue injection. (f) Composite photoacoustic B-scan image corresponding to the dotted line in (d). SK, skin surface. (g) Evans blue dynamics in mice SLNs. Data points were fitted exponentially. PA, photoacoustic.

Image of FIG. 4.
FIG. 4.

Noninvasive in vivo photoacoustic mapping and dynamic monitoring of the sentinel lymph node in a rat. (a) Control photoacoustic MAP image acquired at laser wavelength before Evans blue injection. (b) Control photoacoustic MAP image acquired at , showing the subcutaneous vasculature. (c) Photoacoustic MAP image acquired at after Evans blue injection. LVv, lymphatic valve; SLN, sentinel lymph node. (d) Photoacoustic MAP image acquired at after Evans blue injection. (e) Composite photoacoustic MAP image. (f) Composite photoacoustic B-scan image corresponding to the dotted line in (c). SK, skin surface. (g) Evans blue dynamics in the rat lymphatic valve and SLN.

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/content/aapm/journal/medphys/36/8/10.1118/1.3168598
2009-07-13
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: High-speed dynamic 3D photoacoustic imaging of sentinel lymph node in a murine model using an ultrasound array
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/36/8/10.1118/1.3168598
10.1118/1.3168598
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