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Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis
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10.1118/1.4747269
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    Affiliations:
    1 Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
    2 Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China
    3 Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
    4 Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China
    5 Imaging Unit - Integrative Oncology Department, British Columbia Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada
    a) Authors to whom correspondence should be addressed. Electronic addresses: syfeng@fjnu.edu.cn and chenr@fjnu.edu.cn; Telephone: +86-591-83489919; Fax: +86-591-83465373.
    Med. Phys. 39, 5664 (2012); http://dx.doi.org/10.1118/1.4747269
/content/aapm/journal/medphys/39/9/10.1118/1.4747269
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/39/9/10.1118/1.4747269

Figures

Image of FIG. 1.
FIG. 1.

The UV-visible absorption spectra of RNA (curve a), pure Ag sol (curve d), the mixture of Ag colloidal and RNA (curve c), and the mixture of Ag colloidal, MgSO4, and RNA solution (curve b). The inserted picture shows SEM images of MgSO4-aggregated Ag colloids on the surface of aluminum substrate.

Image of FIG. 2.
FIG. 2.

(a) Comparison of the normalized mean spectra of serum RNA samples obtained from 31 gastric cancer patients versus 34 healthy volunteers. The shaded areas represent the standard deviations (SD) of the means spectra. The stripe shadow represents the standard deviations of the gastric cancer mean spectra, while the gray shaded area indicates the standard deviations of the normal mean spectra. Also shown at the bottom is the difference spectrum. (b) Comparison of the mean intensities and standard deviations of selected peaks with the most distinguishable differences between gastric cancer and normal serum RNA.

Image of FIG. 3.
FIG. 3.

(a) Principal component analysis of all serum RNA samples: a 3D mapping of the PCA result for gastric caner and healthy volunteer group. (b) LDA scores: gastric cancer versus healthy subject. The solid line represents the classification cut-value (y = 0.3130).

Image of FIG. 4.
FIG. 4.

The ROC curve of the discrimination result for using PCA-LDA-based SERS spectral classification with leave-one-out, cross validation method. The integrated area under the ROC curve was 0.989.

Tables

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TABLE I.

SERS peak positions and vibrational mode assignments.

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/content/aapm/journal/medphys/39/9/10.1118/1.4747269
2012-08-29
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis
http://aip.metastore.ingenta.com/content/aapm/journal/medphys/39/9/10.1118/1.4747269
10.1118/1.4747269
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