No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
Perspectives for imaging single protein molecules with the present design of the European XFEL
7. H. Chapman, “ Coherent imaging with x-ray free electron lasers,” in Scattering Methods for Condensed Matter Research: Towards l Applications at Future Sources, Lecture Notes of the 3rd IFF Spring School ( Forschungszentrum Juelich, 2012), Vol. 43.
8. S. Baradaran et al., “ LCLS-II new instruments workshops report,” (2012), see Sec. 4.3.2., p. 60 by Chapman et al. and Sec. 4.3.3., p. 69 by F. R. N. C. Maia et al.
14. A. Mancuso et al., “ Scientific instrument single particles, clusters, and biomolecules (SPB) CDR,” Technical Report No. TR-2011-007, XFEL.EU, 2011.
15. A. Mancuso et al., “ The single particles, clusters and biomolecules (SPB) instrument TDR,” Technical Report No. TR-2013-004, XFEL.EU, 2013.
17. P. Emma, M. Borland, and Z. Huang, “ Attosecond x-ray pulses in the LCLS using the slotted foil method,” in Proceedings of the FEL Conference, Trieste, Italy (2004), Paper No. TUBIS01, p. 333.
21. G. Geloni et al., “ Wake monochromator in asymmetric and symmetric Bragg and Laue geometry for self-seeding the European XFEL,” Technical Report No. DESY 13-013, 2013.
29. G. Geloni
, V. Kocharyan
, and E. Saldin
, “ Scheme for generation of fully-coherent, TW power level hard X-ray pulses from baseline undulators at the European X-ray FEL
,” preprint arXiv:1007.2743
[physics.acc-ph] Report No. DESY 10-108 (2010
30. G. Geloni
, V. Kocharyan
, and E. Saldin
, “ Production of transform-limited X-ray pulses through self-seeding at the European X-ray FEL
,” preprint arXiv:1109.5112
[physics.acc-ph] Report No. DESY 11-165 (2011
31. W. Fawley et al., “ Toward TW-level LCLS radiation pulses,” in Talk at International FEL Conference, Shanghai, China Report No. DESY 14-137 (2011).
32. J. Wu et al., “ Simulation of the hard x-ray self-seeding FEL at LCLS,” in Talk at International FEL Conference, Shanghai, China (2011).
35. H. Sinn et al., “ X-ray optics and beam transport, conceptual design report,” Technical Report No. TR-2011-002, XFEL.EU, 2011.
36. The limitations in aperture arise from (a) the limited length of super-polished mirrors, where 950 mm is already longer than any mirror of that quality that has been delivered to date and (b) the constraint on reflection angle that is limited by the ablation thresholds of suitable mirror coatings.
40. S. Serkez et al.
, “ Perspectives of imaging of single protein molecules with the present design of the European XFEL. Part I. X-ray source, beamline optics and instrument simulations
,” preprint arXiv:1407.8450
42. It should be noted that in practice the minimum sample to detector distance is limited to about 130 mm.
43. Moltrans is a in-house DESY code written by E. Weckert. It is available on request to the author.
Article metrics loading...
The Single Particles, Clusters and Biomolecules & Serial Femtosecond Crystallography (SPB/SFX) instrument at the European XFEL is located behind the SASE1 undulator and aims to support imaging and structure determination of biological specimen between about 0.1 μm and 1 μm size. The instrument is designed to work at photon energies from 3 keV up to 16 keV. Here, we propose a cost-effective proof-of-principle experiment, aiming to demonstrate the actual feasibility of a single molecule
diffraction experiment at the European XFEL. To this end, we assume self-seeding capabilities at SASE1 and we suggest to make use of the baseline European XFEL accelerator complex—with the addition of a slotted-foil setup—and of the SPB/SFX instrument. As a first step towards the realization of an actual experiment, we developed a complete package of computational tools for start-to-end simulations predicting its performance. Single biomolecule imaging capabilities at the European XFEL can be reached by exploiting special modes of operation of the accelerator complex and of the SASE1 undulator. The output peak power can be increased up to more than 1.5 TW, which allows to relax the requirements on the focusing efficiency of the optics and to reach the required fluence without changing the present design of the SPB/SFX instrument. Explicit simulations are presented using the 15-nm size RNA Polymerase II molecule as a case study. Noisy diffraction patterns were generated and they were processed to generate the 3D intensity distribution. We discuss requirements to the signal-to-background ratio needed to obtain a correct pattern orientation. When these are fulfilled, our results indicate that one can achieve diffraction without destruction with about 0.1 photons per Shannon pixel per shot at 4 Å resolution with 1013
photons in a 4 fs pulse at 4 keV photon energy and in a 0.3 μm focus, corresponding to a fluence of 1014 photons/μm2. We assume negligible structured background. At this signal level, one needs only about 30 000 diffraction patterns to recover full 3D information. At the highest repetition rate manageable by detectors at European XFEL, one will be able to accumulate these data within a fraction of an hour, even assuming a relatively low hit probability of about a percent.
Full text loading...
Most read this month