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High resolution multiple excitation spot optical microscopy
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/content/aip/journal/adva/1/2/10.1063/1.3598413
2011-05-27
2014-08-21

Abstract

We propose fundamental improvements in three-dimensional (3D) resolution of multiple excitation spot optical microscopy. The excitation point spread function (PSF) is generated by two interfering counter-propagating depth-of-focus beams along the optical axis. Detection PSF is obtained by coherently interfering the emitted fluorescent light (collected by both the objectives) at the detector.System PSF shows upto 14-fold reduction in focal volume as compared to confocal, and almost 2-fold improvement in lateral resolution. Proposed PSF has the ability to simultaneously excite multiple 3D-spots of sub-femtoliter volume. Potential applications are in fluorescence microscopy and nanobioimaging.

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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: High resolution multiple excitation spot optical microscopy
http://aip.metastore.ingenta.com/content/aip/journal/adva/1/2/10.1063/1.3598413
10.1063/1.3598413
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