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Assessment of the efficacy of laser hyperthermia and nanoparticle-enhanced therapies by heat shock protein analysis
2. H. J. Wei, D. Xing, G. Y. Wu, Y. Jin, and H. M. Gu, “Optical properties of human normal small intestine tissue determined by Kubelka-Munk method in vitro,” World Journal of Gastroenterology 9(9), 2068–2072 (2003).
3. J. Liu, Z. Ren, and C. Wang, “Interpretation of 1iving tissue's temperature oscillations by thermal wave theory,” Chinese Science Bulletin 40, 1493–1494 (1995).
4. M. Heike, B. Noll, and K. H. Meyer zum Büschenfelde, “Heat shock protein-peptide complexes for use in vaccines,” Journal of Leukocyte Biology 60(2), 153–158 (1996).
5. Y. Sakaguchi, L. C. Stephens, M. Makino et al. “Apoptosis in tumors and normal tissues induced by whole body hyperthermia in rats,” Cancer research 55(22), 5459–5464 (1995).
7. M. J. Schlesinger, “Heat shock proteins,” Journal of Biological Chemistry 265(21), 12111–12114 (1990).
8. A. M. Ciupitu, M. Petersson, K. Kono, J. Charo, and R. Kiessling, “Immunization with heat shock protein 70 from methylcholanthrene-induced sarcomas induces tumor protection correlating with in vitro T cell responses,” Cancer Immunol Immunother 51, 163–170 (2002).
11. J. Nylandsted, M. Rohde, K. Brand et al., “Selective depletion of heat shock protein 70 (Hsp70) activates a tumor-specific death program that is independent of caspases and bypasses Bcl-2,” Proceedings of the National Academy of Sciences 97(14), 7871–7876 (2000).
15. P. K. Srivastava, A. B. DeLeo, and L. J. Old, “Tumor rejection antigens of chemically induced sarcomas of inbred mice,” Proceedings of the National Academy of Sciences of the United States of America 83(10), 3407–3411 (1986).
16. M. C. Roccheri, K. Onorato, C. Tipa et al., “EGTA treatment causes the synthesis of heat shock proteins in sea urchin embryos,” Mol Cell Biol Res Commun 3(5), 306–311 (2000).
18. L. Zhongming and L. Xiaohua, “Photobiological foundation and optimum parameter of laser thermotherapy,” Laser Journal 23(23), 67–69 (2002).
19. J. A. Barnes, D. J. Dix, B. W. Collins, C. Luft, and J. W. Allen, “Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia,” Cell Stress Chaperones 6, 316–325 (2001).
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Tumor thermotherapy is a method of cancer treatment wherein cancer cells are killed by exposing the body tissues to high temperatures. Successful clinical implementation of this method requires a clear understanding and assessment of the changes of the tumor area after the therapy. In this study, we evaluated the effect of near-infrared laser tumor thermotherapy at the molecular, cellular, and physical levels. We used single-walled carbon nanotubes (SWNTs) in combination with this thermotherapy. We established a mouse model for breast cancer and randomly divided the mice into four groups: mice with SWNT-assisted thermotherapy; mice heat treated without SWNT; mice injected with SWNTs without thermotherapy; and a control group. Tumors were irradiated using a near-infrared laser with their surface temperature remaining at approximately 45 °C. We monitored the tumor body growth trend closely by daily physical measurements, immunohistochemical staining, and H&E (hematoxylin-eosin) staining by stage. Our results showed that infrared laser hyperthermia had a significant inhibitory effect on the transplanted breast tumor, with an inhibition rate of 53.09%, and also significantly reduced the expression of the heat shock protein Hsp70. Furthermore, we have found that protein analysis and histological analysis can be used to assess therapeutic effects effectively, presenting broad application prospects for determining the effect of different treatments on tumors. Finally, we discuss the effects of SWNT-assisted near-infrared laser tumor thermotherapy on tumor growth at the molecular, cellular, and physical levels.
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