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1.R. Yuste, Nature methods 2(12), 902-904 (2005).
2.L. Shang, K. Nienhaus, X. Jiang, L. Yang, K. Landfester, V. Mailander, T. Simmet, and G. U. Nienhaus, Beilstein journal of nanotechnology 5, 2388-2397 (2014).
3.WHO, 2007.
4.E. J. Arts and D. J. Hazuda, Cold Spring Harbor perspectives in medicine 2(4), a007161 (2012).
5.D. S. Fierer and M. E. Klotman, Current opinion in HIV and AIDS 1(2), 115-120 (2006).
6.T. W. Chun, D. C. Nickle, J. S. Justement, J. H. Meyers, G. Roby, C. W. Hallahan, S. Kottilil, S. Moir, J. M. Mican, J. I. Mullins, D. J. Ward, J. A. Kovacs, P. J. Mannon, and A. S. Fauci, The Journal of infectious diseases 197(5), 714-720 (2008).
7.M. Horiike, S. Iwami, M. Kodama, A. Sato, Y. Watanabe, M. Yasui, Y. Ishida, T. Kobayashi, T. Miura, and T. Igarashi, Virology 423(2), 107-118 (2012).
8.H. K. Makadia and S. J. Siegel, Polymers 3(3), 1377-1397 (2011).
9.C. J. Destache, T. Belgum, M. Goede, A. Shibata, and M. A. Belshan, The Journal of antimicrobial chemotherapy 65(10), 2183-2187 (2010).
10.G. Sahay, D. Y. Alakhova, and A. V. Kabanov, Journal of controlled release : official journal of the Controlled Release Society 145(3), 182-195 (2010).
11.T. Bose, D. Latawiec, P. P. Mondal, and S. Mandal, Journal of Nanoparticle Research 16(8), 1-25 (2014).
12.L. Shang, K. Nienhaus, and G. U. Nienhaus, Journal of Nanobiotechnology 12(5), (2014).
13.H. Fessi, F. Puisieux, J. P. Devissaguet, N. Ammoury, and S. Benita, International Journal of Pharmaceutics 55(1), R1-R4 (1989).
14.R. L. McCall and R. W. Sirianni, Journal of visualized experiments : JoVE (82), 51015 (2013).
15.R. Vijayalakshmi, Vadapalli S. H. Naveena, J. Philip, and M. D. Dhanaraju, Der Pharma Chemica 6(1), 404-406 (2014).
16.S. Ghosh, A. Roy, D. Banik, N. Kundu, J. Kuchlyan, A. Dhir, and N. Sarkar, Langmuir : the ACS journal of surfaces and colloids 31(8), 2310-2320 (2015).
17.G. Wang, Y. Gong, F. J. Burczynski, and B. B. Hasinoff, Free radical research 42(5), 435-441 (2008).
18.A. A. Date, A. Shibata, P. Bruck, and C. J. Destache, Biomedical chromatography : BMC 29(5), 709-715 (2015).
19.D. Ripamonti and F. Maggiolo, Current opinion in investigational drugs (London, England : 2000) 9(8), 899-912 (2008).
20.G. J. Zaharatos and M. A. Wainberg, Annals of medicine 45(3), 236-241 (2013).
21.F. Danhier, N. Lecouturier, B. Vroman, C. Jerome, J. Marchand-Brynaert, O. Feron, and V. Preat, Journal of controlled release : official journal of the Controlled Release Society 133(1), 11-17 (2009).
22.M. M. Yallapu, B. K. Gupta, M. Jaggi, and S. C. Chauhan, Journal of colloid and interface science 351(1), 19-29 (2010).
23.D. Lawrence, B. Sanders, and O. K. Sharma, in NIH AIDS Reagent Program, Beerse, Belgium (2014).
24.P. P. Mondal and A. Diaspro, in Fundamentals of Fluorescence Microscopy, edited byP. P. Mondal and A. Diaspro (Springer, 2014), Vol. 1, pp. 149-159.
25.J. A. Ankrum, O. R. Miranda, K. S. Ng, D. Sarkar, C. Xu, and J. M. Karp, Nature protocols 9(2), 233-245 (2014).
26.A. Bloom and N. Winograd, Surface and interface analysis : SIA 46(Suppl 1), 177-180 (2014).
27.R. Agarwal, V. Singh, P. Jurney, L. Shi, S. V. Sreenivasan, and K. Roy, Proceedings of the National Academy of Sciences of the United States of America 110(43), 17247-17252 (2013).

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In the last decade, confocal fluorescence microscopy has emerged as an ultra-sensitive tool for real-time study of nanoparticles (NPs) fate at the cellular-level. According to WHO 2007 report, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) is still one of the world’s major health threats by claiming approximately 7,000 new infections daily worldwide. Although combination antiretroviral drugs (cARV) therapy has improved the life-expectancy of HIV-infected patients, routine use of high doses of cARV has serious health consequences and requires complete adherence to the regimen for success. Thus, our research goal is to fabricate long-acting novel cARV loaded poly(lactide-co-glycolic acid) (PLGA) nanoparticles (cARV-NPs) as drug delivery system. However, important aspects of cARV-NPs that require special emphasis are their cellular-uptake, potency, and sustained drug release efficiency over-time. In this article, ultra-sensitive confocal microscopy is been used to evaluate the uptake and sustained drug release kinetics of cARV-NPs in HeLa cells. To evaluate with the above goal, instead of cARV-drug, Rhodamine6G dye (fluorescent dye) loaded NPs (Rho6G NPs) have been formulated. To correlate the Rhodamin6G release kinetics with the ARV release from NPs, a parallel HPLC study was also performed. The results obtained indicate that Rho6G NPs were efficiently taken up at low concentration (<500 ng/ml) and that release was sustained for a minimum of 4 days of treatment. Therefore, high drug assimilation and sustained release properties of PLGA-NPs make them an attractive vehicle for cARV nano-drug delivery with the potential to reduce drug dosage as well as the number of drug administrations per month.


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