Skip to main content

News about Scitation

In December 2016 Scitation will launch with a new design, enhanced navigation and a much improved user experience.

To ensure a smooth transition, from today, we are temporarily stopping new account registration and single article purchases. If you already have an account you can continue to use the site as normal.

For help or more information please visit our FAQs.

banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
1.Partha P. Mondal and Alberto Diaspro, Book: Fundamentals of Fluorescence Microscopy, 1st ed. (Springer, 2014).
2.J. R. Lakowicz, Book: Principles of Fluorescence Spectroscopy, 3rd ed. (Springer, 2006).
3.S. T. Hess, T. P. Giriajan, and M. D. Mason, Biophys. Jl. 91, 4258 (2006).
4.E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1642 (2006).
5.M. J. Rust, M. Bates, and X. Zhuang, Nature Methods 3, 793 (2006).
6.S. W. Hell and J. Wichmann, Optics Letters 19, 780 (1994).
7.M. G. Gustafsson, Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081 (2005).
8.J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, and S. W. Hell, Nature Methods 5, 943 (2008).
9.T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, PNAS 106, 22287 (2009).
10.A. Sharonov and R. M. Hochstrasser, Proc. Natl. Acad. Sci. USA 103, 1891118916 (2006).
11.G. Giannone, E. Hosy, F. Levet, A. Constals, K. Schulze, A. I. Sobolevsky, M. P. Rosconi, E. Gouaux, R. Tampé, D. Choquet, and L. Cognet, Biophys. J. 99, 13031310 (2010).
12.M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, Nature Methods 5, 527 (2008).
13.F. C. Zanacchi, Z. Lavagnino, M. P. Donnorso, A. D. Bue, L. Furia, M. Faretta, and A. Diaspro, Nature Meth. 8, 1047 (2011).
14.J. White, A. Helenius, and M. J. Gething, Nature 300, 658 (1982).
15.Manasa V. Gudheti, Nikki M. Curthoys, Travis J. Gould, Dahan Kim, Mudalige S. Gunewardene, Kristin A. Gabor, Julie A. Gosse, Carol H. Kim, Joshua Zimmerberg, and Samuel T. Hess, Biophys. J. 104, 2182 (2013).
16.H. P. Kao and A. S. Verkman, Biophys. J. 67, 1291 (1994).
17.B. Huang, W. Q. Wang, M. Bates, and X. W. Zhuang, Science 319, 810 (2008).
18.H. Deschout, F. C. Zanacchi, M. Mlodzianoski, A. Diaspro, J. Bewersdorf, S. T. Hess, and K. Braeckmans, Nature Meth. 11, 253 (2014).
19.A. Kechkar, D. Nair, M. Heilemann, D. Choquet, and J. B. Sibarita, PLoS ONE 8, e62918 (2013).
20.S. Wolter, M. Schuttpelz, M. Tscherepanow, S. Van de Linde, M. Heilemann et al., J. Microsc 237, 12 (2010).
21.R. Henriques, M. Lelek, E. F. Fornasiero, F. Valtorta, C. Zimmer et al., Nat Methods 7, 339 (2010).
22.S. A. Jones, Sang-Hee Shim, J. He, and X. Zhuang, Nature Methods 8, 499 (2011).
23.A. Furstenberg and M. Heilemann, Phys. Chem. Chem. Phys. 15, 14919 (2013).
24.S. T. Hess, A. A. Heikal, and W. W. Webb, J. Phys. Chem. B 108, 10138 (2004).
25.Partha P. Mondal, Front. Mol. Biosci. 1, 11 (2014).

Data & Media loading...


Article metrics loading...



We propose clean localization microscopy (a variant of fPALM) using a molecule filtering technique. Localization imaging involves acquiring a large number of images containing single molecule signatures followed by one-to-one mapping to render a super-resolution image. In principle, this process can be repeated for other z-planes to construct a 3Dimage. But, single molecules observed from off-focal planes result in false representation of their presence in the focal plane, resulting in incorrect quantification and analysis. We overcome this with a single molecule filtering technique that imposes constraints on the diffraction limited spot size of single molecules in the image plane. Calibration with sub-diffraction size beads puts a natural cutoff on the actual diffraction-limited size of single molecules in the focal plane. This helps in distinguishing beads present in the focal plane from those in the off-focal planes thereby providing an estimate of the single molecules in the focal plane. We study the distribution of actin (labeled with a photoactivatable CAGE 552 dye) in NIH 3T3 mouse fibroblast cells.


Full text loading...


Access Key

  • FFree Content
  • OAOpen Access Content
  • SSubscribed Content
  • TFree Trial Content
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd