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The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision
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/content/aip/journal/apl/100/15/10.1063/1.3700446
2012-04-09
2014-10-20

Abstract

The double-helix point spread function microscope encodes the axial (z) position information of single emitters in wide-field (x,y) images, thus enabling localization in three dimensions (3D) inside extended volumes. We experimentally determine the statistical localization precision σ of this approach using single emitters in a cell under typical background conditions, demonstrating σ < 20 nm laterally and <30 nm axially for N ≈ 1180 photons per localization. Combined with light-induced blinking of single-molecule labels, we present proof-of-concept imaging beyond the optical diffraction limit of microtubule networkstructures in fixed mammalian cells over a large axial range in three dimensions.

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Scitation: The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/15/10.1063/1.3700446
10.1063/1.3700446
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