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Separation of blood cells using hydrodynamic lift
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Image of FIG. 1.
FIG. 1.

Geometry of the microfluidic device and schematic illustration of cell trajectories. Large arrows indicate fluid flow. Note that cells separate with respect to their position in z (height).

Image of FIG. 2.
FIG. 2.

Micrographs of red blood cells and blood platelets at the beginning (left images) and the end (right images) of the microchannel (, ). The graphs below show the corresponding statistics that have been determined by relative counts in intervals of . Below , no platelets are visible due to optical interference of the channel wall. A Gaussian curve has been fitted to the histogram to calculate the average height of the objects. Its standard deviation has been identified as a measure for the scattering of the height values.

Image of FIG. 3.
FIG. 3.

Adopted height against flow rate for RBCs, platelets, and microspheres at and a dynamic viscosity of (a) and (b). An average of about 250 particles has been counted for each species per data point. The dashed lines represent the results of the calculation.

Image of FIG. 4.
FIG. 4.

Separation and sorting of red blood cells (upper channel) and blood platelets (lower channel). The RBC trajectory is visualized by an overlay of 11 consecutive frames with time intervals of 1 ms.


Generic image for table
Table I.

Properties of red blood cells, blood platelets, and polystyrene microspheres.23–30

Generic image for table
Table II.

Adopted heights of red blood cells, blood platelets, and microspheres at with an estimated entrance height of .


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Separation of blood cells using hydrodynamic lift