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(Color online) Representative multiphoton images of the normal (a) and precancerous (b) epithelial tissues. Scale bar = 20 μm.
Population density of nuclei versus percentage of enlarge nuclei obtained from 9 normal (circles) and precancerous (triangles) epithelial tissues pairs.
(Color online) Typical multiphoton images of precancerous epithelial tissues before (a) and after (b) laser processing. Scale bar = 20 μm.
(Color online) Three-dimensional reconstruction of precancerous epithelial tissues after laser processing. Serial optical sections of tissues were obtained to create cross-sectional images in the X-Y, Y-Z, and X-Z planes, showing an ablation depth of 34 μm.
Quantitative characterization parameters derived from multiphoton imaging. PLN: Percentage of large nuclei (the percentage of nuclei larger than across 5 μm). PD: Population density (the total nuclear number per unit area).
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