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Label-free multiphoton imaging and photoablation of preinvasive cancer cells
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10.1063/1.3676271
/content/aip/journal/apl/100/2/10.1063/1.3676271
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/2/10.1063/1.3676271

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Representative multiphoton images of the normal (a) and precancerous (b) epithelial tissues. Scale bar = 20 μm.

Image of FIG. 2.
FIG. 2.

Population density of nuclei versus percentage of enlarge nuclei obtained from 9 normal (circles) and precancerous (triangles) epithelial tissues pairs.

Image of FIG. 3.
FIG. 3.

(Color online) Typical multiphoton images of precancerous epithelial tissues before (a) and after (b) laser processing. Scale bar = 20 μm.

Image of FIG. 4.
FIG. 4.

(Color online) Three-dimensional reconstruction of precancerous epithelial tissues after laser processing. Serial optical sections of tissues were obtained to create cross-sectional images in the X-Y, Y-Z, and X-Z planes, showing an ablation depth of 34 μm.

Tables

Generic image for table
Table I.

Quantitative characterization parameters derived from multiphoton imaging. PLN: Percentage of large nuclei (the percentage of nuclei larger than across 5 μm). PD: Population density (the total nuclear number per unit area).

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/content/aip/journal/apl/100/2/10.1063/1.3676271
2012-01-10
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Label-free multiphoton imaging and photoablation of preinvasive cancer cells
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/2/10.1063/1.3676271
10.1063/1.3676271
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