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Silicon-based reproducible and active surface-enhanced Raman scattering substrates for sensitive, specific, and multiplex DNA detection
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10.1063/1.3701731
/content/aip/journal/apl/100/20/10.1063/1.3701731
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/20/10.1063/1.3701731
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Figures

Image of FIG. 1.
FIG. 1.

SEM (a) and AFM (b) images of the prepared AgNPs@Si. The AFM image is collected from a 5.0 × 5.0 μm2. Raman mapping spectra (c) and corresponding Raman intensity (d) of R6G dispersed on surface of the AgNPs@Si. (λexcitation = 633 nm, acquisition time = 1 s, laser power = 20 mW, hole = 1000, slit = 100, grating = 600, and filter = D2).

Image of FIG. 2.
FIG. 2.

Schematic representation for constructing the silicon-based SERS sensor and its use for DNA detection.

Image of FIG. 3.
FIG. 3.

(a) SERS spectra of target DNA with different concentrations obtained from the silicon-based sensor. (b) DNA is quantitatively detected by measuring the SERS signals of R6G attached to DNA. Raman signals of DNANC and background are presented as control. (c) Detection of single-based mismatches. Raman intensity is challenged with 1 pM DNA target, mutated to either A, G, or C. (d) SERS spectra recorded from 25 random points on the substrates assembled with 10 pM target DNA. (λexcitation = 633 nm, laser power = 20 mW, acquisition time = 1 s, hole = 1000, slit = 100, grating = 600, and filter = D2).

Image of FIG. 4.
FIG. 4.

A scheme for the design of the mixed DNA-functionalized AgNPs@Si (a) and the corresponding SERS spectra for multiplex detection of target DNA (1 pM) (b). (λexcitation = 633 nm, laser power = 20 mW, acquisition time = 1 s, hole = 1000, slit = 100, grating = 600, and filter = D2).

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/content/aip/journal/apl/100/20/10.1063/1.3701731
2012-05-14
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Silicon-based reproducible and active surface-enhanced Raman scattering substrates for sensitive, specific, and multiplex DNA detection
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/20/10.1063/1.3701731
10.1063/1.3701731
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