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(a) Averaged bacteria swimming speed with respect to sodium ion concentration, in 10% glycerol added TMN motility buffer medium. (b) Typical V. alginolyticus motion. The bacterium changes its swimming direction by an almost instantaneous large angle “flick” motion. From top to bottom: the bacterium moves leftwards, then flicks its flagellum at large angle, changes its body orientation, and swims rightwards. (c) The schematic plot (upper row) and the corresponding top view (lower row) of the experiment setup. The PS bead is located 10 µm above the bacterial carpet surface.
(a) The typical 6 min trajectories for various cases. Case A is the reference trajectory for non-bacteria attached microfluidics channel, B-F cases are trajectories for 0, 10, 30, 40, and 50 mM Na+, respectively. A-F cases are all in TMN motility buffer medium added with 10% glycerol. G and H cases are in 50 mM Na+ medium with 5% and 0% glycerol added. (b) Rc for each respective case (B-F), all with 10% glycerol. (c) Mean square displacement for various cases (B, D, F, G, H).
(a) Typical 10 s trajectories for fluorescent labeled 0.2 µm PS particles attached on respective flagellum of a pair of bacteria located in close proximity. Insets show the snapshot and the corresponding definition for its position projection in two dimensional (r, θ) plane. (b) Typical time series for the projection evolution of two neighboring particle in the bacteria pair. (c) The corresponding phase velocity (ω) time series for the angle evolution of the projected trajectory. (d) The corresponding digitized time series for flicking “event”, with the definition of Ta , Tb , and Tab .
The abundance of waiting time interval P(Tb ) for the case H and D, plotted together for comparison. Insets are the normalized histograms of event abundance for the time interval Ta , Tb , and Tab in respective cases.
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