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High resolution resonant waveguide grating imager for cell cluster analysis under physiological condition
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10.1063/1.4723691
/content/aip/journal/apl/100/22/10.1063/1.4723691
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/22/10.1063/1.4723691
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Figures

Image of FIG. 1.
FIG. 1.

EGF induced responses of quiescent A431 cells (>95% confluency). (a) A schematic drawing of the RWG imager. The images of a single biosensor bearing A431 cells: (b) basal resonant wavelength (λbr) and (c) light microscopic image. (d) The real time kinetic responses at 166 pixels across the biosensor (indicated by the white dotted line in (b)), wherein I, II, and III indicate three time points for data analysis. (e) The λbr distribution of the biosensor. Distribution of the EGF responses at 2 min (f), and 9 min and 60 min poststimulation (g). Correlation of the EGF responses at 2 min (h) and 60 min (i) poststimulation with the λbr values at corresponding pixels. The bin size was 100 pm for all histograms.

Image of FIG. 2.
FIG. 2.

Comparison of DMR signals between ambient (23 °C) and physiological (37 °C) conditions. (a)–(d) The DMR of quiescent A431 cells responding to 32 nM EGF (a), 2 μM niacin (b), 2 μM histamine (c), and 100 nM bradykinin (d). (e) and (f) The dose DMR responses of HEK293 cells responding to acetylcholine under ambient and physiological conditions, respectively. (g) and (h) The maximal amplitudes (g), or the amplitudes at 50 min poststimulation (h), as a function of acetylcholine doses. Data represent mean ± s.d. from 2 independent measurements (n = 4). Cell confluency was >95% for all.

Image of FIG. 3.
FIG. 3.

EGF induced responses of A431 cells at low confluency (about 10%). (a) The resonant wavelength images (i–iv), and its corresponding DMR images (v–viii) of a portion of a biosensor having relatively high cell coverage. (b) Distribution of its λbr with a bin size of 100 pm. (c) and (d) Correlation of the EGF responses at 2 min (c) and 60 min (d) poststimulation with its λbr values at corresponding locations. (e) and (f) Representative light microscopic images of A431 before and 1 h after stimulation with 32 nM EGF at 37 °C, wherein a physical mark (the irregular area at the right and bottom sides) was made to define the location for imaging. Arrows point to the cells moved after stimulation.

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/content/aip/journal/apl/100/22/10.1063/1.4723691
2012-05-29
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: High resolution resonant waveguide grating imager for cell cluster analysis under physiological condition
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/22/10.1063/1.4723691
10.1063/1.4723691
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