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Graphene-protein bioelectronic devices with wavelength-dependent photoresponse
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10.1063/1.3678024
/content/aip/journal/apl/100/3/10.1063/1.3678024
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/3/10.1063/1.3678024
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Figures

Image of FIG. 1.
FIG. 1.

(Color online) AFM of monolayer graphene before (a) and after (b) functionalization with His-tagged protein G (1 μm scale bar for both images); z-scales are 8 and 20 nm, respectively), (c) height linescans indicated on (a) and (b). (d) Schematic of chemical coupling between graphene (bottom) and the protein’s histidine tag (top).

Image of FIG. 2.
FIG. 2.

(Color online) Current-gate voltage characteristic (I-VG) of graphene FET after each step required for functionalization with fusion protein GST-BT5: as prepared (black), after diazonium treatment (red dashed), after Ni-NTA attachment (green dotted), and after incubation in protein (GST-BT5) solution (blue dot-dash). Bias voltage is 1 mV. (inset) I-VG characteristics of a GFP-GFET with different illumination conditions: no illumination (black), illumination at 405 nm (violet data), 532 nm (green data), and 632 nm (red data). Illumination intensity is approximately 70 mW/cm2 for each wavelength. Bias voltage is 10 mV.

Image of FIG. 3.
FIG. 3.

(Color online) Photocurrent responses (%ΔI/I) of GFET hybrids incorporating green and yellow fluorescent protein (GFP and YFP, respectively) are determined by the proteins’ optical absorption spectra. Responses are shown to illumination at 405 nm (violet data), 532 nm (green data), and 632 nm (red data). (a) Responses of GFP-GFET. Beginning at time 100 s, the sample is illuminated for 50 s and then the light is turned off for 50 s. Only violet illumination causes a detectable response. Inset: measured absorption spectrum of GFP. The wavelengths used in the experiments are indicated by appropriately colored dots. (b) Photocurrent responses of YFP-GFET hybrid. Beginning at time 50 s, the sample is illuminated for 50 s and then the light is turned off for 50 s. Now only green illumination produces a detectable response. Inset: measured absorption spectrum of YFP with dots indicating wavelengths used.

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/content/aip/journal/apl/100/3/10.1063/1.3678024
2012-01-18
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Graphene-protein bioelectronic devices with wavelength-dependent photoresponse
http://aip.metastore.ingenta.com/content/aip/journal/apl/100/3/10.1063/1.3678024
10.1063/1.3678024
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