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Optical planar waveguide for cell counting
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View: Figures


Image of FIG. 1.
FIG. 1.

(Color online) (a) Schematic side view showing buried planar waveguide. (b) Attenuation of transmitted signals is expected to be proportional to the number of metal particles above. (c) Laboratory bench-top setup showing fiber-coupled laser, waveguide, and photodetector.

Image of FIG. 2.
FIG. 2.

(Color online) (a) Elemental analysis looking at potassium concentration across the surface of the glass (green scan line) shows enrichment in the waveguide stripes (highlighted with white dashed lines). Gray line, measured potassium concentration in arbitrary units. Blue line, smoothed fit of gray line. (b) Elemental analysis showed that potassium was enriched comparing the shallow buried waveguide and bulk glass. Perspective view shows the cut face and the waveguide face (facing downwards), and the zoomed view shows the two rectangular regions interrogated along the cut face. The histogram shows the elemental analysis, revealing only a difference in potassium. (c) Scanning along the cut edge from the surface into the bulk glass, potassium enrichment extended ∼6.5 μm and was noted to be buried at a shallow depth (∼300 nm) as compared with the surface. Gray line, measured potassium concentration in arbitrary units. Blue line, smoothed fit of gray line.

Image of FIG. 3.
FIG. 3.

(Color online) (a) Simulation of waveguide power in the absence of simulated captured “cells.” (b) Waveguide geometry with 100 simulated captured “cells.” (left) is the geometry with the simulated metalized 4 μm × 4 μm captured “cells.” (middle) is the contour map of the field and (right) shows the steadily decreasing power in the waveguide in the region of the interaction with the “cells.”

Image of FIG. 4.
FIG. 4.

(Color online) (a) Faux cells fabricated from reflowed photoresist and coated with aluminum, SEM image and close up. (b) Faux cells were fabricated in a grid pattern over the optical waveguide where surface area coverage was varied by the density of the cells. (c) Laser light was measured after transmission through each waveguide underneath the patterned faux cells. Transmitted power is shown in relation to the coverage of faux cells lying above the waveguide.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Optical planar waveguide for cell counting