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Working principle of the luminescent 1DPC for label-free sensing employing a reversibly sealed microfluidic circuit.
Calculated angular-resolved reflectance spectra of the 1DPC illuminated in the Kretschmann configuration showing the BSW dispersion curve (left); angularly-dependent near-field intensity calculated at 10 nm from the 1DPC surface upon plane-wave illumination at λ = 532 nm (inset); sketch of the layer sequence constituting the 1DPC in contact with the aqueous solution (right).
Normalized measured angularly resolved luminescence spectra of the 1DPC upon BSW laser-excitation. The emitted luminescence is prism-leaking according to the BSW dispersion curve and detected by means of a fiber-based spectrometer. Integration time is 10 s per spectrum, and laser power is 10 mW.
(a-e) Real-time monitoring of the fluorescence peak position as the refractive index of the aqueous medium is perturbed as indicated. Rising and falling ramps are dependent on the flow velocity (kept constant) and diffusion of glucose molecules within the flow cell. Note the different scale of y-axis and the increasing signal to noise ratio with increasing Δn; (f) calculated (blue circles) and measured (red points) sensitivity response ΔλBSW(Δn) obtained for bulky refractive index perturbations Δn of the aqueous medium in contact to the 1DPC surface.
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