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(a) Bright field image of the nanoplasmonic substrate. The square region contains the nanostructure. (b) Angular (30°) SEM image of the nanostructure region after 80 nm Ag deposition. (c) Schematic diagram of FLIM experimental set-up. (d) Schematic of lifetime distribution of dyes ( , are the amplitudes and are the lifetimes) on nanoplasmonic substrate. Here, “d” is the distance of the dye from the nanoplasmonic substrate. (e) Sketch of polar plot and lifetime distribution for the population of dyes on nanoplasmonic substrate. Here, is the phase corresponding to lifetime component.
(a) Absorbance and fluorescence spectra for Rh-B in aqueous solution. (b) Emission spectra of the Rh-B on the nanoplasmonic substrate. (c) Fluorescence intensity image of Rh-B on the nanoplasmonic substrate. The increase is fluorescence intensity on the nanoplasmonic substrate (square region) compared to surrounding smooth metal (Ag) region is apparent. (d) Polar plot representation of lifetime for Rh-B in free solution (Petri Dish) and on nanoplasmonic substrate (Nanopillar). (e) Comparison of the fluorescence spectroscopy result for Rh-B on nanoplasmonic substrate and on glass substrate.
(a) Fluorescence phase lifetime image of Rh-B on nanoplasmonic substrate. (b) Fluorescence modulation lifetime images of Rh-B on the nanoplasmonic substrate. (c) Histogram for lifetime measurement of Rh-B in free solution. (d) Histogram for lifetime measurement of Rh-B on nanoplasmonic substrate.
Descriptions of life time simulations. (a) The principle components of the distributions of lifetimes are displayed as a function of fractional intensities. An exponentially decreasing set of lifetimes as function of fractional intensities (far left panel) is combined with Gaussian distributions (middle two panels) each representing the smooth-silver and nanopillar separately to determine the final distribution (far right panel). (b) A three dimensional view of the general profile of the simulated distributions is presented on the polar plot. The color hue represents an increasing fractional intensity (blue to red). (c) These measured polar coordinates were applied to the simulations to establish the locations of the nanopillar and smooth silver film distributions. Background was factored in separately.
Fluorescence lifetime analysis: , fluorescence intensity on the nanoplasmonic structure; , fluorescence intensity on glass substrate; EF, fluorescence enhancement factor.
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