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(a) Schematic of the spatial-filtering based quantitative phase imaging system. LAS: laser; CL: condenser lens; SC: sample chamber; MO: microscope objective; M1-3: mirrors; BS1 and 2: beam splitter; PH: pin hole. (b) Pseudo-colored (color bar in right) quantitative phase map of a 6 μm polystyrene microsphere surrounded by immersion oil.
(a) Interference pattern of HEK cells in isotonic solution. Inset: zoomed view of the interference pattern. (b) Wrapped phase image. (c)Unwrapped phase image of the cells in isotonic solution. (d) Unwrapped phase image after 2 min in 150 mOsm/Kg hypotonic stimulation. The hypotonic stimulation is marked by dark band. (e) Kinetics of change in phase profile of the HEK cells due to hypotonic stimulation. Also shown is the backgrond phase values.
(a) Schematic time-sequence of swelling of cell and change in intra-cellular refractive index (displayed in gray scale) subjected to hypotonic stimulation. (b) Decrease in phase value of an HEK cell as a function of time after hypotonic stimulation. The 150 mOsm/Kg hypotonic stimulation is applied at 0 s. (c) Increase in phase value of the HEK cell as a function of time after ∼100 s of hypotonic stimulation. Also shown are fits to using polynomial equations Ф1 = A1 + B1 t + C1 t2 and Ф2 = A2 + B2 t + C2 t2 for graphs in (b) and (c), respectively. (d) Contour plot for Morlet wavelet coefficients with scale and time. The X-time scale is 1250 points over 500 s. The color bar represents amplitude of the coefficient of wavelets of different scales. (e) Variation of Hurst exponent (h) with moment (q) of a series.
The values for mean and standard deviation of parameters (B1,C1) and (B2, C2) obtained from 6 cells.
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