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Multicolor in vivo brain imaging with a microscope-coupled fiber-bundle microprobe
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10.1063/1.4767386
/content/aip/journal/apl/101/23/10.1063/1.4767386
http://aip.metastore.ingenta.com/content/aip/journal/apl/101/23/10.1063/1.4767386
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Figures

Image of FIG. 1.
FIG. 1.

Diagram of multicolor brain imaging with a microscope-coupled fiber-bundle microprobe: GM, galvanometrically scanned mirrors; Obj, objective; L1, L2, lenses; F1, F2, F3, filters; PMT, photomultipliers.

Image of FIG. 2.
FIG. 2.

Typical images of neuron circuits in the brain of a Thy1-EGFP mouse obtained through a fiber bundle coupled to a confocal microscope by using the fluorescence response from EGFP with the distal end of the fiber probe immersed at different depth in a fixed brain sample: (a) a raw image delivered by the fiber, (b) an image of the same area after Gaussian filtering with σ = 2.5 μm, (c) a stack of images obtained with a fiber-bundle microprobe immersed at different depths inside a fixed brain tissue, and (d) a typical image of individual neurons in the brain tissue obtained with a standard confocal microscope. The scale bar in the images is 50 μm.

Image of FIG. 3.
FIG. 3.

Two-color images of dentate gyrus in the hippocampus of a transgenic Thy-1-EGFP mice additionally stained with DAPI dilactate acquired in a living mouse with the use of the fiber-bundle microprobe (a) and with fixed brain slices from the same mouse by means of confocal microscopy (b). The scale bar is 100 μm (a) and 50 μm (b).

Image of FIG. 4.
FIG. 4.

Three-color image of dentate gyrus in the hippocampus of a transgenic Thy-1-EGFP mice double-stained with DAPI dilactate and Neurotrace Nissl 640/660 dye obtained with the use of the fiber-bundle microprobe. The scale bar is 100 μm.

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/content/aip/journal/apl/101/23/10.1063/1.4767386
2012-12-04
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Multicolor in vivo brain imaging with a microscope-coupled fiber-bundle microprobe
http://aip.metastore.ingenta.com/content/aip/journal/apl/101/23/10.1063/1.4767386
10.1063/1.4767386
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