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Study of radiation effects on the cell structure and evaluation of the dose delivered by x-ray and -particles microscopy
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Image of FIG. 1.
FIG. 1.

X-ray fluorescence maps of several elements from a single PC12 cell. Color scale bar indicates minimum and maximum content of a given element in the sample (in ). The colors are scaled to the maximum of each map. Scanning size was 200 × 201 pixels.

Image of FIG. 2.
FIG. 2.

Phase contrast images of the PC12 cell presented in Fig. 1. Left images correspond to data taken before the fluorescence scan, while the right ones were acquired after the fluorescence scan. White line along the cell indicates an area taken for the measurement of structure differences shown on the profile plots.

Image of FIG. 3.
FIG. 3.

Indirect comparison between STIM (a) and phase contrast image (b) of the irradiated cell. Dashed white line indicates the square burnt out during the fluorescence scan. For better comparison, the contrast was enhanced and does not represent the full range of the images. Scales of the images are slightly different, and the width of a phase image is 47 μm.


Generic image for table
Table I.

Dose delivered to the cell during different experiments and time of acquiring data.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Study of radiation effects on the cell structure and evaluation of the dose delivered by x-ray and α-particles microscopy