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(a) Experiment setup: BS1,2, beam splitters; BB1,2, beam blockers; L1,100× oil immersion objective lens; L2, achromatic tube lens; M0-M3, silver coated mirrors; SP, sample plane; IP, image plane; and CCD, charge-coupled device camera; (b) Spectrum of the combined (three-laser) signal obtained using an Ocean Optics spectrometer. The three insets highlight the laser peaks; (c) RGB spectral response of a typical three-color CCD camera. (Manufacturer data: Lumenera Infinity1-2 C, http://www.emsdiasum. com/microscopy/technical/datasheet/95000.pdf)
Single-shot spectroscopic phase measurement from a 6 -μm polystyrene bead in immersion oil. (a–c) Interferogram images of the polystyrene bead when each laser (780-nm, 532-nm, and 405-nm) is turned ON one at a time; (d–f) Corresponding FFT images (800 × 600 pixels) of the interferograms shown in (a–c); (g and h) Interferogram of the same bead and corresponding FFT image when all three lasers are ON at the same time; (i–k) Quantitative phase images of the same bead at the three wavelengths, 780-nm, 532-nm, and 405-nm, respectively, obtained from the single interferogram illustrated in (g). Scale bar: 1.5 μm.
Dispersion curve for water at 20 °C (from Ref. 21) and that for the cell culture medium (DMEM), based on our measured RI values (indicated with green circles) and Cauchy’s equation.
(a) Three-color single-shot interferogram image and (b–d) the corresponding quantitative phase images of an RKO human colon cancer cell at the three wavelengths. Scale bar: 5 μm; (e) Average refractive indices of 10RKO cells at the three wavelengths using the spherical-cell assumption.23
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