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Flow-based structured illumination
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View: Figures


Image of FIG. 1.
FIG. 1.

Experimental setup. A wide, deep fluidic channel is at the focal plane of a objective lens. The imaging system has low numerical aperture (NA = 0.1). The structured illumination source is a steady sinusoidal-profile ( stripes) orthogonal to the flow direction. Images are recorded on a CCD camera at a frame rate 15 fps.

Image of FIG. 2.
FIG. 2.

Three consecutive frames of two yeast particles under structured illumination. The constant phase shift ( ) of the illumination pattern between consecutive frames shows the evolution of the recorded Moiré pattern.

Image of FIG. 3.
FIG. 3.

Numerical reconstruction of yeast particles. (a) The image under uniform illumination (without SI). (b) Fourier spectra (log scale) of (a). (c) Reconstructed yeast particles using SI, calculated with the three consecutive images of Figs. 2(a)–2(c) and Eq. (4) . (d) Fourier spectra (log scale) of (c). ((e) and (f)) Line profiles of the cross-sections shown in ((a) and (c)) and ((b) and (d)), respectively.

Image of FIG. 4.
FIG. 4.

Schematic of 2D structured illumination.

Image of FIG. 5.
FIG. 5.

Numerical reconstruction using two-dimensional illumination. (a) The image under uniform illumination (without SI). ((b) and (c)) Reconstruction using (b) one-dimensional (1D) and (c) two-dimensional (2D) illumination pattern.

Image of FIG. 6.
FIG. 6.

Trade-off between resolution improvement and the robustness of reconstruction. (a) Equivalent radius ratio (dotted blue line) and magnitude of determinant of (solid black line) versus chosen angle. (b) Alignment error versus reconstruction error.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Flow-based structured illumination