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Normalized autocorrelation curves for AuNP (R = 2.5 nm) diffusing in BSA solution in phosphate buffer at various protein concentrations. Solid lines are fit to the curves using Eq. (2) . Arrow shows direction of increasing concentration.
Diffusion coefficient of R = 2.5 nm AuNPs as a function of protein concentration. The inset shows the measured diffusion for 5 and 10 nm AuNPs at higher concentrations of BSA. Also shown (stars) viscosity as a function of BSA concentration.
Hydrodynamic radii of NPs plotted as a function of BSA concentration. Red solid line represents fit of anticooperative binding model, and blue dashed line shows comparison to Langmuir binding isotherm fitted to first and last 30% of data points. The conversion of concentration units is as follows: [BSA]g/ml = [BSA]μM × Mw × 10−9, where Mw is the molecular weight of BSA and is equal to 66 430 g/mol. The inset shows KD as a function of the hydrodynamic radius Rh.
Translational diffusion coefficient (D) of AuNPs obtained by autocorrelation analysis, and hydrodynamic radius (Rh) calculated using SE relation in absence and presence of BSA.
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