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Schematic of the integrated quantitative phase microscopy and optical manipulation setup. TL, BE, M1 & 2: Steering mirrors, DM, MO, IL, BS1&2, L: Lens, HAL, and CL.
Optical tweezers assisted quantitative phase mapping of rotated RBC. (a) Bright-field image of discotic RBCs, with one RBC (marked by arrow) oriented vertically by optical tweezers (40 mW). (b) Image showing interference fringes, (c) corresponding wrapped phase image. (d–l) Unwrapped quantitative phase image of the RBCs, showing return of the oriented RBC to horizontal plane (enhanced online). [URL: http://dx.doi.org/10.1063/1.4812985.1]doi: 10.1063/1.4812985.1.
(a) Topographic image of the RBC obtained using optical tweezers assisted QPM method. (b) Height maps of the discotic RBC along the lines drawn on (a).
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