No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
Three-dimensional imaging of biological cells with picosecond ultrasonics
15. E. Strohm and M. Kolios, in Engineering in Medicine and Biology Society, EMBC 2009, Annual International Conference of the IEEE (2009), pp. 6042–6045.
20. C. Rossignol, N. Chigarev, M. Ducousso, B. Audoin, G. Forget, F. Guillemot, and M. C. Durrieu, Appl. Phys. Lett. 93, 123901 (2008).
21. M. Ducousso, C. Rossignol, B. Audoin, F. Guillemot, and M. C. Durrieu, in 2008 IEEE Ultrasonics Symposium (IUS) ( IEEE, 2008), pp. 574–577.
25. T. Dehoux, M. A. Ghanem, O. F. Zouani, M. Ducousso, N. Chigarev, C. Rossignol, N. Tsapis, M.-C. Durrieu, and B. Audoin, Ultrasonics 56, 160 (2015).
35. H. N. Lin, R. J. Stoner, H. J. Maris, J. Harper, C. Cabral, J. M. Halbout, and G. W. Rubloff, Appl. Phys Lett. 61, 2700 (1992).
38.The slight horizontal banding is thought to be an artifact of the signal acquisition and interpolation.
39. G. A. Truskey, J. S. Burmeister, E. Grapa, and W. M. Reichert, J. Cell Sci. 103, 491 (1992).
43.The range of validity of the WT data was verified by synthetic sinusoidal signals: 200–600 ps and 200–800 ps for the endothelial and fat cells, respectively.
44. A. Najmi and J. Sadowsky, J. Hopkins APL Tech. D 18, 134 (1997).
46. G. D. Fullerton and J. A. Zagzebski, Medical Physics of CT and Ultrasound: Tissue Imaging and Characterization, Medical Physics Monograph ( The American Institute of Physics, 1980).
49. T. K. Kari and J. V. Matti, Handbook of Hydraulic Fluid Technology ( Marcel Dekker, Inc., 1999).
Article metrics loading...
We use picosecond ultrasonics to image animal cells
in vitro—a bovine aortic endothelial cell and a mouse adipose cell—fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with ∼1 μm lateral and ∼150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli.
Full text loading...
Most read this month