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Cannula-based computational fluorescence microscopy
15. V. M. Patel and R. Chellappa, Sparse Representations and Compressed Sensing for Imaging and Vision ( Springer Science & Business Media, New York, 2013).
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We converted a solid-glass cannula into a high-resolution widefield fluorescence microscope. Calibrating the space-variant point-spread functions of the cannula and applying a nonlinear optimization algorithm to reconstruct object details enable this development. The resolution of our system is ∼1 μm, and fluorophore position is determined to a precision of ∼20 nm. Images of microglia from fixed slices of mouse brains at various post-natal development stages were also obtained.
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