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/content/aip/journal/apl/106/26/10.1063/1.4923402
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/content/aip/journal/apl/106/26/10.1063/1.4923402
2015-07-01
2016-12-08

Abstract

We converted a solid-glass cannula into a high-resolution widefield fluorescence microscope. Calibrating the space-variant point-spread functions of the cannula and applying a nonlinear optimization algorithm to reconstruct object details enable this development. The resolution of our system is ∼1 m, and fluorophore position is determined to a precision of ∼20 nm. Images of microglia from fixed slices of mouse brains at various post-natal development stages were also obtained.

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