Skip to main content

News about Scitation

In December 2016 Scitation will launch with a new design, enhanced navigation and a much improved user experience.

To ensure a smooth transition, from today, we are temporarily stopping new account registration and single article purchases. If you already have an account you can continue to use the site as normal.

For help or more information please visit our FAQs.

banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
K. Suhling, L. M. Hirvonen, J. A. Levitt, P.-H. Chung, C. Tregidgo, A. Le Marois, D. A. Rusakov, K. Zheng, S. Ameer-Beg, S. Poland, S. Coelho, R. Henderson, and N. Krstajic, Med Photonics 27, 3 (2015).
Advanced Time-Correlated Single Photon Counting Applications, edited by W. Becker ( Springer International Publishing, Switzerland, 2015).
G. S. Buller and R. J. Collins, Meas. Sci. Technol. 21, 012002 (2010).
R. H. Hadfield, Nat. Photonics 3, 696 (2009).
W. Becker, Advanced Time-Correlated Single Photon Counting Techniques ( Springer, Berlin, Heidelberg, 2005).
A. Esposito, T. Oggier, H. Gerritsen, F. Lustenberger, and F. Wouters, Opt. Express 13, 9812 (2005).
M. Raspe, K. M. Kedziora, B. van den Broek, Q. Zhao, S. de Jong, J. Herz, M. Mastop, J. Goedhart, T. W. Gadella, I. T. Young, and K. Jalink, Nat. Methods 13, 501 (2016).
Q. Zhao, B. Schelen, R. Schouten, R. van den Oever, R. Leenen, H. van Kuijk, I. Peters, F. Polderdijk, J. Bosiers, M. Raspe, K. Jalink, J. G. S. de Jong, B. van Geest, K. Stoop, and I. T. Young, J. Biomed. Opt. 17, 126020 (2012).
H. Chen, G. Holst, and E. Gratton, Microsc. Res. Tech. 78, 1075 (2015).
E. Gratton, S. Breusegem, J. Sutin, Q. Ruan, and N. Barry, J. Biomed. Opt. 8, 381 (2003).
Z. Petrášek, H.-J. Eckert, and K. Kemnitz, Photosynth. Res. 102, 157 (2009).
J. S. Lapington, Nucl. Instrum. Methods Phys. Res., Sect. A 525, 361 (2004).
X. Michalet, R. A. Colyer, G. Scalia, A. Ingargiola, R. Lin, J. E. Millaud, S. Weiss, O. H. Siegmund, A. S. Tremsin, J. V. Vallerga, A. Cheng, M. Levi, D. Aharoni, K. Arisaka, F. Villa, F. Guerrieri, F. Panzeri, I. Rech, A. Gulinatti, F. Zappa, M. Ghioni, and S. Cova, Philos. Trans. R. Soc. B 368, 20120035 (2013).
J. Vallerga, J. McPhate, A. Tremsin, and O. Siegmund, Astrophys. Space Sci. 320, 247 (2009).
S. A. Stern, D. C. Slater, J. Scherrer, J. Stone, G. Dirks, M. Versteeg, M. Davis, G. R. Gladstone, J. W. Parker, L. A. Young, and O. H. W. Siegmund, Space Sci. Rev. 140, 155 (2008).
V. Emiliani, D. Sanvitto, M. Tramier, T. Piolot, Z. Petrášek, K. Kemnitz, C. Durieux, and M. Coppey-Moisan, Appl. Phys. Lett. 83, 2471 (2003).
G. Giraud, H. Schulze, T. T. Bachmann, C. J. Campbell, A. R. Mount, P. Ghazal, M. R. Khondoker, A. J. Ross, S. W. J. Ember, I. Ciani, C. Tlili, A. J. Walton, J. G. Terry, and J. Crain, Int. J. Mol. Sci. 10, 1930 (2009).
O. H. W. Siegmund, M. Lampton, R. Raffanti, and W. Herrick, Nucl. Instrum. Methods Phys. Res., Sect. A 310, 311 (1991).
J. Milnes, J. S. Lapington, O. Jagutzki, and J. Howorth, Nucl. Instrum. Methods Phys. Res., Sect. A 604, 218 (2009).
O. Jagutzki, J. S. Lapington, L. B. C. Worth, U. Spillman, V. Mergel, and H. Schmidt-Böcking, Nucl. Instrum. Methods Phys. Res., Sect. A 477, 256 (2002).
D. M. Owen, C. Rentero, A. Magenau, A. Abu-Siniyeh, and K. Gaus, Nat. Protoc. 7, 24 (2012).
M. Liu, M. Jia, H. Pan, L. Li, M. Chang, H. Ren, F. Argoul, S. Zhang, and J. Xu, Appl. Spectrosc. 68, 577 (2014).
P. R. Barber, S. M. Ameer-Beg, J. Gilbey, L. M. Carlin, M. Keppler, T. C. Ng, and B. Vojnovic, J. R. Soc., Interface 6, S93 (2009).
C. Tregidgo, J. A. Levitt, and K. Suhling, J. Biomed. Opt. 13, 031218 (2008).
J. V. Vallerga and J. B. McPhate, Proc SPIE 4139, 34 (2000).
C. C. Davis and T. A. King, J. Phys. A: Gen. Phys. 3, 101 (1970).
O. Jagutzki, A. Cerezo, A. Czasch, R. Dörner, M. Hattaß, M. Huang, V. Mergel, U. Spillmann, K. Ullmann-Pfleger, T. Weber, H. Schmidt-Böcking, and G. Smith, IEEE Nucl. Sci. Symp. Conf. Rec. 2, 850 (2001).
L. M. Hirvonen, Z. Petrášek, A. Beeby, and K. Suhling, New J. Phys. 17, 023032 (2015).
L. M. Hirvonen, F. Festy, and K. Suhling, Opt. Lett. 39, 5602 (2014).
A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d'Este, S. Jakobs, C. Eggeling, and S. W. Hell, Nat. Methods 10, 737 (2013).
L. Lanzano, I. Coto Hernandez, M. Castello, E. Gratton, A. Diaspro, and G. Vicidomini, Nat. Commun. 6, 6701 (2015).
X. Michalet, R. Colyer, J. Antelman, O. Siegmund, A. Tremsin, J. Vallerga, and S. Weiss, Curr. Pharm. Biotechnol. 10, 543 (2009).

Data & Media loading...


Article metrics loading...



We perform wide-field time-correlated single photon counting-based fluorescence lifetime imaging (FLIM) with a crossed delay line anode image intensifier, where the pulse propagation time yields the photon position. This microchannel plate-based detector was read out with conventional fast timing electronics and mounted on a fluorescence microscope with total internal reflection (TIR) illumination. The picosecond time resolution of this detection system combines low illumination intensity of microwatts with wide-field data collection. This is ideal for fluorescence lifetime imaging of cell membranes using TIR. We show that fluorescence lifetime images of living HeLa cells stained with membrane dye di-4-ANEPPDHQ exhibit a reduced lifetime near the coverslip in TIR compared to epifluorescence FLIM.


Full text loading...


Access Key

  • FFree Content
  • OAOpen Access Content
  • SSubscribed Content
  • TFree Trial Content
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd