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Experimental and theoretical scaling laws for transverse diffusive broadening in two-phase laminar flows in microchannels
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5.The high initial concentration of (1 mM) relative to that of fluo-3 (5 M) makes our fluorescence measurement much more sensitive to diffusion of than to that of fluo-3, and increases the asymmetry of the diffusion profile. We added EDTA (4 M) to the solution of fluo-3 in order to reduce the background fluorescence and increase contrast by scavenging opportunistic metal ions that could bind to fluo-3.
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