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Alignment of filamentous proteins and associated molecules through confinement in microchannels
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View: Figures


Image of FIG. 1.
FIG. 1.

(Color online) (a) Schematic drawing of the F-actin/-actinin bundle structure. The F-actin and -actinin form a 2D distorted square lattice. (b) Fluorescence optical micrograph of actin bundled with -actinin showing the network of bundles.

Image of FIG. 2.
FIG. 2.

(a) Schematic layout of the microchannel device. (b) SEM image of a section of the device; (c) fluorescence micrograph of actin bundled with -actinin inside a single -channel. The bundles are predominantly aligned parallel to the channel.

Image of FIG. 3.
FIG. 3.

(Color online) (a) 2D SAXS data from a bulk sample (in a quartz capillary) of the F-actin/-actinin complex. The diffuse ring of scattering intensity at , resulting from the F-actin/-actinin structure shown in Fig. 1, shows partial alignment in the sample. (b) 2D SAXS pattern from an F-actin/-actinin sample prepared in the microchannel device. The diffraction peaks are strongly aligned along the meridional direction, which is perpendicular to the channel. (c) Azimuthal scans taken at the first-order peak position from the two diffraction patterns demonstrate the dramatically increased alignment in the sample prepared in the microchannel. (d) scan taken along the oriented diffraction data in (b) shows three peaks (marked by arrows) which index to the distorted square lattice of the F-actin/-actinin bundle.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Alignment of filamentous proteins and associated molecules through confinement in microchannels