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SEM photographs of the silicon pyramids having an octagonal shape due to the etching of the  planes in silicon and with a top diagonal of 2 μm.
Schematic of the press setup emphasizing the pneumatic drive and the drive chuck, which bears the silicon matrix on its free end. The drive base is mounted on the probe-station platen (not shown), whereas the plastic slide was fixed by vacuum mounting on the stage chuck of the wafer prober.
Inverted pyramids on the plastic surface after pressing the silicon matrix chip on the slide. (a) Single inverted pyramid elongated at its inverted top and with a protruding base. (b) Two arrays of inverted pyramids embossed on the slide after two sequential printing steps (with a 50 μm pitch in the and dimension).
(Color) (a) Fluorescence picture of the rabbit -globulins and biotinylated-BSA spot arrays after a 2 h immunoreaction with a mixture of AF 546 labeled streptavidin (red spots) and AF 488 labeled antirabbit IgG antibody (green spots). The composite picture shown is the digital superposition of the two individual pictures taken with the red and the green emission filters, respectively. (b) Plot of the interleaved red and green signal intensities. Assay conditions: Blocking solution: BSA solution in 0.1 M carbonate buffer, pH 8.5. Probe immobilization buffer: 50 mM carbonate buffer, pH 9.2. Bioreaction buffer: 50 mM Tris-HCl, pH 7.8, containing 9 g of NaCl, 10 g of BSA, 0.05 g of bovine -globulins, and 0.05 g of .
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