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Protein patterning by micromachined silicon embossing on polymer surfaces
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10.1063/1.1841458
/content/aip/journal/apl/85/26/10.1063/1.1841458
http://aip.metastore.ingenta.com/content/aip/journal/apl/85/26/10.1063/1.1841458
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

SEM photographs of the silicon pyramids having an octagonal shape due to the etching of the [331] planes in silicon and with a top diagonal of 2 μm.

Image of FIG. 2.
FIG. 2.

Schematic of the press setup emphasizing the pneumatic drive and the drive chuck, which bears the silicon matrix on its free end. The drive base is mounted on the probe-station platen (not shown), whereas the plastic slide was fixed by vacuum mounting on the stage chuck of the wafer prober.

Image of FIG. 3.
FIG. 3.

Inverted pyramids on the plastic surface after pressing the silicon matrix chip on the slide. (a) Single inverted pyramid elongated at its inverted top and with a protruding base. (b) Two arrays of inverted pyramids embossed on the slide after two sequential printing steps (with a 50 μm pitch in the and dimension).

Image of FIG. 4.
FIG. 4.

(Color) (a) Fluorescence picture of the rabbit -globulins and biotinylated-BSA spot arrays after a 2 h immunoreaction with a mixture of AF 546 labeled streptavidin (red spots) and AF 488 labeled antirabbit IgG antibody (green spots). The composite picture shown is the digital superposition of the two individual pictures taken with the red and the green emission filters, respectively. (b) Plot of the interleaved red and green signal intensities. Assay conditions: Blocking solution: BSA solution in 0.1 M carbonate buffer, pH 8.5. Probe immobilization buffer: 50 mM carbonate buffer, pH 9.2. Bioreaction buffer: 50 mM Tris-HCl, pH 7.8, containing 9 g of NaCl, 10 g of BSA, 0.05 g of bovine -globulins, and 0.05 g of .

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/content/aip/journal/apl/85/26/10.1063/1.1841458
2004-12-17
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Protein patterning by micromachined silicon embossing on polymer surfaces
http://aip.metastore.ingenta.com/content/aip/journal/apl/85/26/10.1063/1.1841458
10.1063/1.1841458
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