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(a) A conventional flow cell consisting of a microscope slide, acid-washed cover slip, and glass spacers all held together by vacuum grease. (b) A modified flow cell with lithographically defined heater (gray, on the exterior of the cover slip) and the thermometer (dark gray stripe, on the interior surface) on the same cover slip for temperature dependent measurements. (c) Typical vs characteristic of a thin narrow-stripe gold thermometer. Sensitivity of the thermometer, , is given in the inset.
Actin filament sliding speed response as a function of time upon slow cyclical heating and cooling for three cycles. Temperature data were also plotted.
Speed of actin filaments as a function of temperature obtained from heating phases of the three cycles shown in Fig. 2. Inset: Arrhenius plot of the data. The inferred activation energy, , was obtained from linear regression analysis. The error is maximum estimated error and is larger than standard error of the regression.
Response in actin filament speed (solid squares) as a function of time upon rapid temperature increase (open circles).
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