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Experimental setup of the CARS microscope; BS: beam splitter; LF: long-wavelength-pass filter; NF: 800-nm Notch filter; SF: short-wavelength-pass filter.
Ultrabroadband multiplex CARS spectra and their delay-time dependence for two solvents, (a) and indene (b). Upper part: spectral profile of the CARS signal at the fixed delay time of 0 (thick solid), −1.2 (dotted), and −0.53 (broken) ps. The CARS signal intensity at 0 ps is scaled by 3. The intensity-corrected CARS spectrum of indene is also shown as a hatched curve, Lower part: Two-dimensional intensity plot of the CARS signal. The vertical and horizontal axis corresponds to delay time and wavelength of the CARS signal, respectively. Darker regions indicate greater signal intensities.
(a) Spectral profile of the CARS signal. The filled curves with dark (1) and light gray (2) correspond to the spectra of a polystyrene bead with a diameter of 3 μm and water, respectively. (b) CARS lateral images of a polystyrene bead at the Raman shift of (left) and (right). Brighter regions indicate greater signal intensities. The CARS spectra (1) and (2) in (a) are obtained at and (−3.1 μm, −3.1 μm), respectively.
(a) CARS spectrum of a multilamellar vesicle (MLV), (b) CARS lateral image of a MLV detected at , which corresponds to the aliphatic C–H stretching vibrational mode. Brighter regions indicate greater signal intensities.
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