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Simple scheme for rapid three-dimensional orientation determination of the emission dipole of single molecules
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10.1063/1.1888040
/content/aip/journal/apl/86/12/10.1063/1.1888040
http://aip.metastore.ingenta.com/content/aip/journal/apl/86/12/10.1063/1.1888040
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) (a) Outline of the detection scheme based on a confocal microscope. (b) Sketch illustrating the dipole emission and involved angles. The cutoff angle is defined by the annular mirror, the rim angle by the numerical aperture of the microscope objective. is the polar angle of the dipole. (c) Relative intensities of the light passing the aperture of the mirror (dashed line) and reflected off the mirror (dotted line) as a function of the polar angle of the emission dipole.

Image of FIG. 2.
FIG. 2.

(Color online) (a) Fluorescence intensity image of single PDI molecules as obtained by scanning the sample and summing up all three signals. False color images of (b) azimuthal angles and (c) the polar angles for the molecules visible in (a) representing the orientation image. (d) Histograms of (dark gray) and (light gray, offset of 60) values for 319 observed molecules.

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/content/aip/journal/apl/86/12/10.1063/1.1888040
2005-03-14
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Simple scheme for rapid three-dimensional orientation determination of the emission dipole of single molecules
http://aip.metastore.ingenta.com/content/aip/journal/apl/86/12/10.1063/1.1888040
10.1063/1.1888040
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