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Probing local water contents of in vitro protein films by ultrasonic force microscopy
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View: Figures


Image of FIG. 1.
FIG. 1.

(a) Theoretical force-distance curve presenting a sum of forces between an AFM tip and a sample during an indentation process (loading curve) or tip-sample separation (unloading curve). The curves for approaching and retracting the sample are different, which is marked by arrows. The hysteresis area corresponds to the adhesion hysteresis. Due to the presence of ultrasound the mean position of an AFM tip changes from the initial indentation (1) through the point (2) with increasing ultrasonic amplitude to reach the point (3) where loss of contact has already occurred. (b) An example of UFM data. are the changes of ultrasonic force at acquired, modulating frequency and are the ultrasonic amplitudes. These are measured just after the jump-up point and just before the jump-up .

Image of FIG. 2.
FIG. 2.

Measured UFM spectra for albumin (alb), concanavalin (con) and uncovered silicon substrate (Si). “HH” means relative humidity of 50% and “LH” refers to less than 5% of relative humidity.

Image of FIG. 3.
FIG. 3.

UFM measured adhesion hysteresis as a function of the LFM friction force for the investigated samples.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Probing local water contents of in vitro protein films by ultrasonic force microscopy