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Printing of DNA molecules on glass. (a) An optical Microscopy image of the microprinted DNA rectangular spots onto microscope slides, using a 248-nm KrF laser system with a 500-fs pulse duration, laser fluence, and 1 pulse per spot, corresponding to a high-density microarray of (b), (c) A Southern blot of lambda phage DNA. The electrophoresis pattern of lambda phage DNA digested by Styl restriction endonuclease. Autoradiography of the blotted lambda phage DNA shown in (b), using the material spotted by laser printing for generating the specific probe (c). pBS is the pBluescript SK phagemid 2958 base pairs and is used as a negative control for the hybridization reaction.
Printing of bovine serum albumin (BSA) protein on glass. A scanning electron microscopy image of the microprinted BSA array of rectangular spots with resolution onto microscope slides, using a 248-nm KrF laser system with a 500-fs pulse duration, laser fluence and 1 pulse per spot.
(Color) Visualizing protein–protein interactions on epoxy-coated glass. (a) Hybrid proteins possessing different epitopes. (b) A fluorescence image of the anti-FLAG–Cy5 indicating the binding of the anti-FLAG protein in both patterns FLAG–GST–6His and FLAG–GST–HA. (c) A fluorescence image of the anti-HA–Alexa 594 indicating the selectively binding of the antibody to epitope HA of the FLAG–GST–HA protein. (d) The fluorescence image of the anti-6His FITC indicating the selective binding of the antibody to epitope 6His of the FLAG–GST–6His protein.
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