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(Color online) (a) Photograph of the microfluidic device. (b) Magnified image of its main functional area. (c) Schematic diagram showing structure of the flow in the device with the liquid injected from port appearing in a dark color.
Confocal micrographs showing distributions of fluorescent dye (FITCD) near the central axis of the test channel at different pressures at the device inlets. (a) and (c)–(f) were taken at the beginning of the test channel and (b) was taken near the end of it. Port was blocked and differential pressures at ports , , , and in kPa with respect to port were: (a), (b) 6.6, 9.7, 7.4, and 9.4; (c) 8.6, 6.9, 9.7, and 10.7; (d) 4.1, 5.9, 3.7, and 9.4; (e) 4.3, 4.6, 4.1, and 11.2; (f) 3.8, 4.8, 3.3, and 11.2.
(a) Fluorescence micrograph of streak lines produced by fluorescent beads focused to thick flow layer. (b) Bright field snapshot of beads focused to thick flow layer. Port was blocked and differential pressures at ports , , , and in kPa with respect to port were: (a) 2.4, 2.0, 2.8, and 2.9; (b) 1.4, 1.0, 1.5, and 1.8.
Fluorescence micrographs of a thick blob of FITCD solution taken with a WI objective with exposure: (a) at the beginning of the test channel, (b) downstream, and (c) downstream (at the end of the channel). Differential pressures at ports , , , and in kPa with respect to port were 29.5, 38.1, 29.5, and 42.2. Differential pressure at port was normally with no flow through port , and in order to create the blob it was dropped to for two times with an interval of .
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