Light-induced cell separation in a tailored optical landscape
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A sample chamber containing of an equal number of lymphocytes and erythrocytes and a Bessel beam with a diameter core is imaged using a microscope objective. 17 (a) Two lymphocytes can be seen in the top left corner, surrounded by erythrocytes. (b) The sample is exposed to the Bessel beam. (c) and (d) Cells are transported towards the central core. (e) The arrangement of cells at the top of the sample chamber with two lymphocytes aligned vertically in the beam center and the erythrocytes, aligned with their largest axis in the direction of beam propagation, in the outer rings of the Bessel beam. (f) Lymphocytes can be extracted from the sample into a separate reservoir using a carefully positioned microcapillary.
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Isolation of CD2 T-lymphocytes from a sample of mononuclear cells. 18 (a) The white and black arrows point to lymphocytes that have been attached to streptavidin-coated microspheres (using a mouse, anti-CD2 monoclonal antibody, and a biotinylated antimouse antibody). The surrounding cells are other mononuclear cells isolated from venous blood. The mixed ensemble of cells is at the bottom of a sample chamber and imaged using a microscope objective. (b)–(e) The sphere-labeled cells travel into the central maximum of the Bessel beam and are guided vertically within the center until they reach the top of the sample chamber where they form a vertical stack (f). Over the time scale required for isolation of the CD2 T-cells (seconds) the unlabeled cells do not move significantly.
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