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Single-pulse cell stimulation with a near-infrared picosecond laser
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10.1063/1.2147733
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    Affiliations:
    1 Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
    2 Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
    3 Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
    4 Department of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
    a) Author to whom correspondence should be addressed; electronic mail: iwanaga@ap.eng.osaka-u.ac.jp
    b) Also at: RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
    Appl. Phys. Lett. 87, 243901 (2005); http://dx.doi.org/10.1063/1.2147733
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View: Figures

Figures

Image of FIG. 1.
FIG. 1.

The energy density dependence of the intracellular photogenerated wave response. The percentage of cells that responded to pulsed laser light was plotted against each energy density of laser. The laser ( wavelength, repetition rate, exposure time) was focused into the cytoplasm of the HeLa cell. Each data point was the average of 25 cells.

Image of FIG. 2.
FIG. 2.

waves induced by focused picosecond laser pulse in a HeLa cell. (a) A series of fluorescence images of an intracellular wave. The laser ( wavelength, energy density, repetition rate, exposure time) was focused into the cytoplasm of the HeLa cell. (b) Plot of dose response relationship with laser energy density. The percentage of cells that responded to pulsed laser light was plotted against each energy density of laser. Filled circles indicate repetition rate and exposure time. Open circles indicate repetition rate and exposure time. Each data point was the average of 20 cells.

Image of FIG. 3.
FIG. 3.

(a) The probability of intracellular wave generation at each energy density. The laser ( wavelength, repetition rate, exposure time) was focused into the cytoplasm of the HeLa cell. The number of laser pulses was adjusted by changing the repetition rate and the exposure time. (b) The energy density required for generating intracellular waves with a probability of 50% at each number of laser pulses.

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/content/aip/journal/apl/87/24/10.1063/1.2147733
2005-12-06
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Single-pulse cell stimulation with a near-infrared picosecond laser
http://aip.metastore.ingenta.com/content/aip/journal/apl/87/24/10.1063/1.2147733
10.1063/1.2147733
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