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Nanofilter array chip for fast gel-free biomolecule separation
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View: Figures


Image of FIG. 1.
FIG. 1.

(a) Layout of the nanofilter array chip. The device includes four buffer access holes (anode, cathode, sample and waste), a 1 cm separation column (a periodic array of nanofilters) and a T-shaped injector. (b) Cross-sectional schematic diagram of the nanofilter array along the separation channel. The nanofilter consists of a thin region and a thick region of equal lengths. The period of one nanofilter is . (c) Scanning electron microscopy images of the cross section of thin regions with different depths (40, 60, 80, and 180 nm).

Image of FIG. 2.
FIG. 2.

Separation of SDS-protein complexes and dsDNA molecules in a nanofilter array device (: 60 nm, : 300 nm, ). Band assignment for SDS-protein complexes: (1) cholera toxin subunit (MW: 11.4 kDa); (2) lectin phytohemagglutinin-L (MW: 120 kDa); (3) low density human lipoprotein (MW: 179 kDa). Band assignment for DNA (PCR marker sample): (1) 50; (2) 150; (3) 300; (4) 500; (5) 766 bp. (a) Sequence of fluorescence images showing separation of the SDS-protein complexes under the electric field of . The solid lines indicate the T-shaped injector and the dashed lines indicate the nanofilter array. The values listed under the images indicate the distance from the injection point. (b) & (c) Separation of SDS-protein complexes and dsDNA molecules under different applied fields. Separation length: 5 mm. : separation resolution between peak and ; , : theoretical plate number and plate height (in microns) for peak ; : theoretical plate number per column length (in ). : electrophoretic mobility of peak .

Image of FIG. 3.
FIG. 3.

Comparison of separation performance in three different nanofluidic chips. Chip1 has only a 60 nm thin, flat channel without any nanofilter. Chip2: , , ; Chip3: , , . Band assignment is the same as in Fig. 2 for SDS-protein complexes. The separation lengths and the applied fields are indicated in the figures.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Nanofilter array chip for fast gel-free biomolecule separation