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Deep-ultraviolet light-emitting diodes for frequency domain measurements of fluorescence lifetime in basic biofluorophores
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10.1063/1.2031934
/content/aip/journal/apl/87/8/10.1063/1.2031934
http://aip.metastore.ingenta.com/content/aip/journal/apl/87/8/10.1063/1.2031934
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Normalized frequency response of the UVTOP® 340 and 280 nm LEDs.

Image of FIG. 2.
FIG. 2.

(a) Normalized fluorescence spectra of NADH (circles) and riboflavin (triangles) under 340 nm LED excitation (solid line). (b) Normalized fluorescence spectra of L-tyrosine (squares) and tryptophan derivative NATA (diamonds) under 280 nm LED excitation (solid line). The LEDs are equipped with shortpass filters to remove residual long-wavelength emission.

Image of FIG. 3.
FIG. 3.

Phase shift (open points) and modulation (solid points) as a function of frequency for (a) NADH, (b) riboflavin, (c) L-tyrosine and (d) tryptophan derivative NATA under 340 nm [(a) and (b)] and 280 nm [(c) and (d)] LED excitation. The lines correspond to single-exponent fluorescence decay modeling with the lifetimes of (a) , (b) , (c) , (d) .

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/content/aip/journal/apl/87/8/10.1063/1.2031934
2005-08-18
2014-04-23
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Deep-ultraviolet light-emitting diodes for frequency domain measurements of fluorescence lifetime in basic biofluorophores
http://aip.metastore.ingenta.com/content/aip/journal/apl/87/8/10.1063/1.2031934
10.1063/1.2031934
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