Full text loading...
(Color online) Converting temporal into spatial information: The upper graph shows the temporal evolution of the fluorescence intensity profile after switching on a Gaussian laser beam illuminating a homogeneous distribution of fluorophores. The photophysical parameters used are: fluorescence lifetime , triplet state lifetime , intersystem crossing rate . Peak excitation rate (at zero position) is assumed to be . The bottom graph shows the fluorescence signal decay as would be measured at the different indicated positions: The higher the excitation intensity, the faster the molecules are pumped into the triplet state and the faster the fluorescence signal approaches its long-time equilibrium level.
(Color online) Point spread function obtained when scanning an infinitely narrow line distribution of fluorescent molecules. Shown are the resulting signals for a diffraction limited focus in the limit of no optical saturation, the same focus with DOSM, and a Bessel focus with DOSM.
(Color online) Demonstration of DSOM performance on a richly structured sample. Top left panel shows the assumed fluorophore distribution (graph of a portrait of microscopy pioneer Antonin van Leuwenhoek), and the length of the black bar is . The top right panel shows an image as obtained when scanning with the diffraction limited focus in the limit of no optical saturation, the bottom left when using DSOM, and the bottom right panel when using DSOM with a Bessel beam focus.
Article metrics loading...