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Detection of nano-confined DNA using surface-plasmon enhanced fluorescence
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10.1063/1.2161813
/content/aip/journal/apl/88/1/10.1063/1.2161813
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/1/10.1063/1.2161813
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Figures

Image of FIG. 1.
FIG. 1.

Typical scanning electron microscope images of the highly uniform AAO self-assembled periodic nanopore array. (a) Cross-sectional view of a -thick membrane deposited on a silicon substrate. (b) Top-surface view.

Image of FIG. 2.
FIG. 2.

(Color online) High resolution structure of DNA-oligomer bis-intercalated with TOTO-1 dimeric cyanine fluorescent dye. (a) High resolution structure of the complex (see Ref. 10). (b) Associated orbital structure of the same complex (see Ref. 9). The DNA bis-intercalator TOTO-1 dye fluoresces exclusively when intercalated in between the DNA helical double strands.

Image of FIG. 3.
FIG. 3.

(Color online) The modified Kretschmann-Raether (ATR) setup for surface-plasmon resonance (SPR)-induced fluorescence measurements.

Image of FIG. 4.
FIG. 4.

(Color online) (a) Attenuation coefficient in reflection due to surface-plasmon polariton resonant coupling as a function of the incident angle for the evaporated metal film on quartz with (엯) and without (●) TOTO-1 bis-intercalated -DNA complexes lying on the Ag surface. (b) Surface-plasmon resonantly coupled light intensity (엯) and the associated 552 nm fluorescence emission intensity (●) as a function of the incident angle for the evaporated metal film on quartz with TOTO-1 bis-intercalated -DNA on the surface.

Image of FIG. 5.
FIG. 5.

(Color online) (a) Comparison of the fluorescence emission of TOTO-1 bis-intercalated -DNA complexes deposited on quartz (엯) and on the Ag film and excited via SPR evanescent-wave coupling (●). (b) SPR-induced fluorescence of intercalated -DNA confined inside the AAO nanopores (●) and lying on a polymer layer (▵). All measurements were performed using a 10 mW incident laser power.

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/content/aip/journal/apl/88/1/10.1063/1.2161813
2006-01-05
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Detection of nano-confined DNA using surface-plasmon enhanced fluorescence
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/1/10.1063/1.2161813
10.1063/1.2161813
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