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Probing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropy
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10.1063/1.2183358
/content/aip/journal/apl/88/10/10.1063/1.2183358
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/10/10.1063/1.2183358
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Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic of the experimental setup. (b) Profile of laser intensity and the diffusion of a fluorescently marked mRNA molecule. (c) Four representative secondary structures obtained by simulation. (d) Typical intensity profile of a fluorescent mRNA diffusing through the confocal volume. (e) Corresponding autocorrelation function of the time series of fluorescence intensity indicating the associated diffusion coefficient.

Image of FIG. 2.
FIG. 2.

(a) Polymerization kinetics of mRNA measured using fluorescence anisotropy. Inset: Bulk anisotropy of labeled mRNA as a function of salt concentration: open circles represent and filled triangles represent . (b) Fluorescence anisotropy time series of three representative mRNA molecules. Inset: corresponding distributions of individual mRNA anisotropy.

Image of FIG. 3.
FIG. 3.

(a) Histogram of fluorescence anisotropy values of mRNA in water (open circles) and (filled circles). (b). Mean anisotropy of mRNA as a function of concentration. The associated standard deviation is also shown. (c) Anisotropy histogram for mRNA in water (open circles) and in RNA hybridization buffer (RHB) (filled squares).

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/content/aip/journal/apl/88/10/10.1063/1.2183358
2006-03-07
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Probing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropy
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/10/10.1063/1.2183358
10.1063/1.2183358
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