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The figure illustrates the principle of the translational velocity measurement for a single cell. The arbitrary component of the spatial frequency is derived from the random refraction index distribution as the periodic light intensity distribution. By observing changes in this light intensity, the translational velocity vector can be measured by a high-response photodiode.
Periodic light intensity distribution is derived from the random refractive index distribution. (a) The gray scale pattern that is created on the PC. As shown in (b), the contrast of a sample surface is low. Moreover, (c) shows the fringe that is formed by interference between the selected component of the special frequency and zeroth-order light. The interval of this fringe is .
This graph shows the relationship between angle of velocity and -component value . By changing the velocity vector from to at intervals of 15°, the -component value is changed in accordance with the cosine law.
The periodic light intensity distribution from a cancerous cell is observed by using a light source of low coherence that does not form the fringe resulting from the interference of stray light. (a) A cell of a human cancer line (diameter: ). As shown in (b), the periodic light intensity distribution can be obtained from the low-contrast cell.
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