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Monitoring cell-cycle-related viscoelasticity by a quartz crystal microbalance
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10.1063/1.2177647
/content/aip/journal/apl/88/8/10.1063/1.2177647
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/8/10.1063/1.2177647
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Figures

Image of FIG. 1.
FIG. 1.

(Color online) Stability test of the QCM set-up along with a schematic representation of the measuring chamber (inset). The plot shows both a stability test in a pure buffer and after FCS insertion (see arrow). after FCS addition, the signal is stable. The principle components of the QCM set-up are (A) cover plate; (B) water reservoir to prevent evaporation in the QCM chamber; (C) contacts for the electrodes; (D) AT-cut crystal; (E) O rings.

Image of FIG. 2.
FIG. 2.

(Color online) Long time monitoring of 50.000 synchronized (77%) cells seeded on the QCM electrode. Bottom inset: Zoom of seeding and spreading phase. Top inset: Fluorescence microscopy image of cells on the QCM electrode at the end of the experiment (scale ).

Image of FIG. 3.
FIG. 3.

A comparison between a synchronized (77% in ) (thicker line) and not-synchronized (60% in ) (thinner line) during the proliferating phase. Whereas a modulation of the frequency signal is clearly evident in the case of the synchronized cells, no modulation is found in the case of nonsynchronized cells.

Image of FIG. 4.
FIG. 4.

Frequency behavior of the QCM for cells treated with colchicine. Arrow 1 points to the seeding of 50.000 cells in the QCM cell. Arrow 2 indicates the time of injection of colchicine into the solution at a final concentration of .

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/content/aip/journal/apl/88/8/10.1063/1.2177647
2006-02-24
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Monitoring cell-cycle-related viscoelasticity by a quartz crystal microbalance
http://aip.metastore.ingenta.com/content/aip/journal/apl/88/8/10.1063/1.2177647
10.1063/1.2177647
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