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3D structure constructed using three-photon polymerization.
(Color online) (a) 3D structure with photolytically attached biotin and streptavidin. The 3D component fluoresces very strongly when exposed to green light. (b) A 3D structure with the photobiotin attachment step omitted. Streptavidin is not attached to the structure and therefore the component does not fluoresce with green light.
Real-time binding of of avidin to photobiotin modified ORMOCER polymer surface, as detected using the SAW technique. The different lines correspond to different photobiotin concentrations.
Effect of photobiotin concentration on the avidin binding.
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