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Construction of three-dimensional biomolecule structures employing femtosecond lasers
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10.1063/1.2359533
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    Affiliations:
    1 Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece and Department of Physics, University of Crete, Crete 71409, Greece
    2 Institute of Molecular Biology and Biotechnology (IMBB), FORTH, Heraklion, Crete 711 10, Greece and Department of Biology, University of Crete, Crete 71409, Greece
    3 Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece and Department of Physics, University of Crete, Crete 71409, Greece
    4 Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece
    5 Institute of Molecular Biology and Biotechnology (IMBB), FORTH, Heraklion, Crete 711 10, Greece and Department of Biology, University of Crete, Crete 71409, Greece
    6 Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece and Department of Physics, University of Crete, Crete 71409, Greece
    7 Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece
    a) Electronic mail: mfarsari@iesl.forth.gr
    Appl. Phys. Lett. 89, 144108 (2006); http://dx.doi.org/10.1063/1.2359533
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View: Figures

Figures

Image of FIG. 1.
FIG. 1.

3D structure constructed using three-photon polymerization.

Image of FIG. 2.
FIG. 2.

(Color online) (a) 3D structure with photolytically attached biotin and streptavidin. The 3D component fluoresces very strongly when exposed to green light. (b) A 3D structure with the photobiotin attachment step omitted. Streptavidin is not attached to the structure and therefore the component does not fluoresce with green light.

Image of FIG. 3.
FIG. 3.

Real-time binding of of avidin to photobiotin modified ORMOCER polymer surface, as detected using the SAW technique. The different lines correspond to different photobiotin concentrations.

Image of FIG. 4.
FIG. 4.

Effect of photobiotin concentration on the avidin binding.

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/content/aip/journal/apl/89/14/10.1063/1.2359533
2006-10-05
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Construction of three-dimensional biomolecule structures employing femtosecond lasers
http://aip.metastore.ingenta.com/content/aip/journal/apl/89/14/10.1063/1.2359533
10.1063/1.2359533
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