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(a) Micro interferometric backscatter detection setup used to monitor the binding events inside the capillary. The beam splitter makes it possible to detect the centroid and the first fringes. The capillary is situated on a temperature controlled stage. (b) Cross-sectional view of the capillary used in both experiments and theoretical work. is the inner radius, . is the outer radius, . is the radius including the polyimid coating, . The insert shows the affinity layer thickness. This layer is modeled as one material with a changing thickness from . (c) Experimental and modeled fringe patterns for the capillary used. The RI of the liquid is set to 1.33. The wave model has been low pass filtered and offset vertically for clarity. The low pass filter is a moving average filter with a window of 0.17°.
Experimentally oberserved changes in the absolute phase in fringe pattern power spectrum (see main text) upon subsequent binding events for extravidin–protein A–human IgG . The signal has been corrected for the different RIs between the buffer solutions.
Fringe positions have been found for both modeled and experimentally obtained fringe patterns.
Ellipsometry data under dry conditions from the biomolecule sandwich fabricated on the silicon wafer. A clear indication of the addition of the extravidin is seen as the thickness increases from . The binding of the IgG fragments does not give rise to any change in thickness, as measured by ellipsometry.
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