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Excitation of fluorescence decay using a pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers
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10.1063/1.2245441
/content/aip/journal/apl/89/6/10.1063/1.2245441
http://aip.metastore.ingenta.com/content/aip/journal/apl/89/6/10.1063/1.2245441

Figures

Image of FIG. 1.
FIG. 1.

Pulse profiles of the LED and IBH 5000F nanosecond flashlamp at their respective and repetition rates under which they were operated.

Image of FIG. 2.
FIG. 2.

(A) Emission spectral profile of LED with bandwidth. The FWHM of the main peak is . (B) Fluorescence spectrum of phenylalanine at of 7.4 excited using the LED. The structure of phenylalanine is also shown.

Image of FIG. 3.
FIG. 3.

LED instrumental pulse, fluorescence decay of phenylalanine ( of 9.2), fitted function, and weighted residuals for a biexponential (fit 2) model. The weighted residuals for a monoexponential fitted function (fit 1) and chi-squared values are shown for comparison.

Tables

Generic image for table
Table I.

Source average and peak output powers. Note that the flashlamp power is integrated between 200 and .

Generic image for table
Table II.

Fluorescence decay times of phenylalanine in water at of 6 and buffered at of 7.4 and 9.2 fitted to monoexponential (1E) and biexponential (2E) models using LED and flashlamp excitations at .

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/content/aip/journal/apl/89/6/10.1063/1.2245441
2006-08-10
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Excitation of fluorescence decay using a 265nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers
http://aip.metastore.ingenta.com/content/aip/journal/apl/89/6/10.1063/1.2245441
10.1063/1.2245441
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