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Buried microfluidic channel for integrated patch-clamping assay
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Image of FIG. 1.
FIG. 1.

Schematics of the patch-clamp recording setup: (a) classical approach where a glass micropipette is used to patch a cell adhered to the bottom of a Petri dish; [(b) and (c)] chip-based approaches where planar and lateral apertures fixed on the substrates are shown to patch a cell suspended in bath solution.

Image of FIG. 2.
FIG. 2.

(Color) [(a)–(d)] Schematics of the substrate after major fabrication steps: (a) etching trench; (b) PSG deposition and trapping void inside the trench; (c) PSG reflow, transforming void into cylindrical shape, and surface planarization; (d) etching chambers on both sides, exposing the lateral patch aperture; (e) scanning electron microscopy (SEM) image of the device with long and diameter buried microchannel; (f) SEM close-up view of the patch aperture.

Image of FIG. 3.
FIG. 3.

(Color) Device under test: (a) before and (b) after capturing a PC12 cell, and (c) magnified view of the fluorescence emission from the captured cell under UV excitation (microchannel length of and diameter of ). The circles mark the location of the patch aperture in the bath chamber which was isolated from the recording chamber by a PDMS capping layer (arrow points at the edge).

Image of FIG. 4.
FIG. 4.

Representative current recordings from a device with long and diameter buried microchannel in response to a voltage stimulus pulse of : (a) before capturing a cell at no bias potential (resistance of ) and (b) after capturing a PC12 cell at a bias potential of (resistance of ).


Generic image for table
Table I.

Measured electrical resistances across different microchannels before and after capturing a PC12 cell.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Buried microfluidic channel for integrated patch-clamping assay